Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants By Jain, Shri Mohan, Saxena, Praveen K. (Eds.)

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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Series: Methods in Molecular Biology, Vol. 547

By Jain, Shri Mohan, Saxena, Praveen K. (Eds.)

2009

Hardcover

 

  • Provides step-by-step protocols for the establishment of in vitro cultures of key medicinal plants and their mass multiplication in a controlled environment
  • Provides step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

Content Level » Professional/practitioner

 

 

Contents

Preface ........................................................................................................................... v
Contributors .................................................................................................................. xi

PART I: MICROPROPAGATION AND CHEMICAL ANALYSIS

1. Establishment of Adventitious Root Cultures of Echinacea
purpurea for the Production of Caffeic Acid Derivatives ........................................ 3
Kee-Yoeup Paek, Hosakatte Niranjana Murthy, and Eun-Joo Hahn

2. In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium,
Assessment of Genetic Fidelity of Micropropagated Plants,
and Simultaneous Quantitation of Reserpine, Ajmaline, and Ajmalicine ................. 17
M.K. Goel, S. Mehrotra, A.K. Kukreja, K. Shanker, and S.P.S. Khanuja

3. Medicinal Properties, In Vitro Protocols
and Secondary Metabolite Analyses of Scots Pine .................................................. 35
Hely Häggman, Anna Maria Pirttilä, Karoliina Niemi,
Tytti Sarjala, and Riitta Julkunen-Tiitto

4. Saussurea medusa Cell Suspension Cultures
for Flavonoid Production ...................................................................................... 53
Chun-Zhao Liu and Praveen K. Saxena

5. Large-scale In Vitro Multiplication of Crataeva nurvala ....................................... 61
Shashi B. Babbar, Neetika Walia, and Amandeep Kaur

6. Bilberry In Vitro Protocols and Analyses
of Phenolic Compounds ....................................................................................... 71
Laura Jaakola, Kaisu Riihinen, Hely Häggman, and Anja Hohtola

7. In Vitro Propagation of Two Tuberous Medicinal Plants:
Holostemma ada-kodien and Ipomoea mauritiana .................................................. 81
S. Pillai Geetha, A.V. Raghu, Gerald Martin, Satheesh George,
and Indira Balachandran

8. In Vitro Production of Gymnemic Acid from Gymnema
sylvestre (Retz) R. Br. Ex Roemer and Schultes Through
Callus Culture Under Abiotic Stress Conditions .................................................... 93
Abdul Bakrudeen Ali Ahmed, Adhikarla Suryanarayana Rao,
and Mandali Venkateswara Rao

9. Establishment of Plant Regeneration and Cryopreservation
System from Zygotic Embryo-Derived Embryogenic Cell
Suspension Cultures of Ranunculus kazusensis ...................................................... 107
Suk Weon Kim and Myung Jin Oh

10. In Vitro Culture and Secondary Metabolite Isolation in Bryophytes ...................... 117
Aneta Sabovljevic, Marko Sabovljevic, and Nebojsa Jockovic
vii

11. Micropropagation and In Vitro Conservation of Vanilla
(Vanilla planifolia Andrews) ................................................................................. 129
Minoo Divakaran and K. Nirmal Babu

12. Protocol for In Vitro Regeneration and Marker Glycoside
Assessment in Swertia chirata Buch-Ham ............................................................. 139
Sushma Koul, K. A. Suri, P. Dutt, M. Sambyal,
A. Ahuja, and M.K. Kaul

13. Protocols for Establishment of an In Vitro Collection
of Medicinal Plants in the Genus Scutellaria ......................................................... 155
Ian B. Cole, Faisal T. Farooq, and Susan J. Murch

14. Protocols for In Vitro Culture and Phytochemical Analysis
of Phyllanthus Species (Euphorbiaceae) ................................................................. 167
Elizabete Catapan, Fábio Netto Moreno, Márcio Luís Busi da Silva,
Michel Fleith Otuki, Rivaldo Niero, Valdir Cechinel Filho,
Rosendo Augusto Yunes, and Ana Maria Viana

15. In Vitro Clonal Propagation of Asparagus racemosus,
a High Value Medicinal Plant ................................................................................ 179
Sanjay Saxena and Nishritha Bopana

16. Micropropagation of Penthorum chinense Through
Axillary Buds ........................................................................................................ 191
Jun Yang and Zheng-song Peng

PART II: TRANSGENIC APPROACHES

17. Spontaneous Plant Regeneration and Production
of Secondary Metabolites from Hairy Root Cultures
of Centaurium erythraea Rafn .............................................................................. 205
Angelina Subotic´ , Slad¯ana Jevremovic´, Dragoljub Grubišic´ ,
and Teodora Jankovic´

18. Transgenic Hypericum perforatum ........................................................................ 217
G. Franklin, Margarida M. Oliveira, and Alberto C.P. Dias

19. Agrobacterium-Mediated Transformation
of Ruta graveolens L. ............................................................................................ 235
Karine Lièvre, Thi Lê Minh Tran, Sébastien Doerper,
Alain Hehn, Paul Lacoste, Brigitte Thomasset,
Frédéric Bourgaud, and Eric Gontier

20. Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures ........... 249
Katarína Brunˇáková and Ján Košuth

21. Catapol Production in Chinese Foxglove (Rehmannia
glutinosa Libos.) Hairy Roots Transformed
with Agrobacterium rhizogenes ATCC15834 ......................................................... 263
Sung Jin Hwang

PART III: MOLECULAR MARKERS AND MICROSATELLITES

22. Identification of Medicinal Plants and Plant Sequences:
A Multiplexed MLPA Assay .................................................................................. 277
Roger A. Barthelson

23. Isolation of Microsatellites from Catharanthus roseus (L.)
G. Don Using Enriched Libraries .......................................................................... 289
Sabhyata Bhatia and Bhumika Shokeen

PART IV: BIOTRANSFORMATION, BIOREACTORS, AND METABOLOMICS

24. Production of Cinnamyl Glycosides in Compact Callus
Aggregate Cultures of Rhodiola rosea Through
Biotransformation of Cinnamyl Alcohol ................................................................ 305
Zsuzsanna György and Anja Hohtola

25. Spearmint Plantlet Culture System as a Means
to Study Secondary Metabolism ............................................................................ 313
Brent Tisserat, Mark Berhow, and Steven F. Vaughn

26. Bioreactor Production of Secondary Metabolites
from Cell Cultures of Periwinkle and Sandalwood ................................................. 325
Jagan V. Valluri

27. Camptothecin Production by In Vitro Cultures and Plant Regeneration
in Ophiorrhiza Species .......................................................................................... 337
Takashi Asano, Hiroshi Sudo, Mami Yamazaki, and Kazuki Saito

28. Metabolomic Analysis of Ocotea odorifera Cell Cultures:
A Model Protocol for Acquiring Metabolite Data.................................................. 347
Marcelo Maraschin, Paulo Fernando Dias, Ênio Luiz Pedrotti,
Hiliana Nunes Ferreira Morais, Ana Maria Viana,
and Karl Vernon Wood

29. The Production of 9-methoxycanthin-6-one from Callus
Cultures of (Eurycoma longifolia Jack) Tongkat Ali ............................................... 359
Mahmood Maziah and Noormi Rosli

PART V: ALTERED GRAVITY AND BIOTECHNOLOGY

30. Plant Secondary Metabolism in Altered Gravity ..................................................... 373
Lindsey K. Tuominen, Lanfang H. Levine, and Mary E. Musgrave

31. The Role of Biotechnology in the Production
of the Anticancer Compound Podophyllotoxin ..................................................... 387
Hemant Lata, Cassia S. Mizuno, and Rita M. Moraes

 

Index ........................................................................................................................... 403

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