Modern Phytochemical Methods by Fischer, Nikolaus H., Isman, Murray B., Stafford, Helen A. (Eds.)

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Modern Phytochemical Methods

Fischer, Nikolaus H., Isman, Murray B., Stafford, Helen A. (Eds.)

Softcover reprint of the original 1st ed. 1991


The past two decades have seen major developments in the methods that can be applied toward the isolation, separation, and structure determination of complex natural products. Modern Phytochemical Methods presents some of the more recent developments of such major separation techniques as high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SF0), and surveys the latest advances in commonly used structure determination methods.

The book commences with thorough examinations of preparative separations of biologically active plant constituents, developments in the prefactionation and semi-preparative separation of free and glycosidically bound volatile natural products by HPLC, and enantio selective separations in phytochemistry. Next, the collection changes its focus to demonstrate how SEC is a valuable complement to HPLC. Among other pertinent issues discussed, chapters review strategies for structure elucidation of natural products; novel chromatography studies with immobilized artificial membranes; commonly used 1 D and 2D NMR techniques; and microsampling methodologies for isolation and analysis of trichrome constituents. A review of quantitative correlations of biological activities of natural products concludes the text.

Phytochemists, plant biochemists, and ecologists will find Modern Phytochemical Methods a useful guide to current concepts in the field.




1. New Developments in the Separation of Natural Products.-

2. Recent Advances in High-Performance Liquid Chromatography of Volatile Natural Products.-

3. Enantioselective Separations in Phytochemistry.-

4. Supercritical Fluid Chromatography for the Analysis of Natural Products.-

5. Immobilized Artificial Membrane Chromatography.-

6. Mass Spectrometry in the Chemistry of Natural Products.-

7. Structure Elucidation of Natural Products by Mass Spectroscopy.-

8. Modern NMR Methods in Phytochemical Studies.-

9. Trichrome Microsampling of Sesquiterpene Lactones for the Use of Systematic Studies.-

10. Biosynthetic Methods for Plant Natural Products: New Procedures for the Study of Glandular Trichrome Constituents.-

11. Quantitative Structure-Activity Relationship Analysis of Natural Products: Phototoxic Thiophenes.



About the Author

Nikolaus H. Fischer Louisiana State University Baton Rouge, Louisiana Murray B. Isman University of British Columbia Vancouver, British Columbia, Canada Helen A. Stafford Reed College Portland, Oregon

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