High Performance Liquid Chromatography in Phytochemical Analysis by Monika Waksmundzka-Hajnos

Share on Whatsapp Share on Whatsapp

High Performance Liquid Chromatography in Phytochemical Analysis



  • Describes the techniques and instrumentation of HPLC analysis, such as gradient elution and computer-assisted methods, HPLC/mass spectrometry, HPLC/nuclear magnetic resonance spectrometry, HPLC with photodiode array detection, HPLC chiral analysis, and much more
  • Discusses how HPLC is used for analyzing herbal drugs and dietary supplements
  • Explores the application of HPLC to primary and secondary plant metabolites, including carbohydrates, lipids, amino acids, peptides, proteins, shikimic acid derivatives, coumarins, flavonoids, lignans, isoprenoids, diterpenes, tripenes, polyterpenes, steroids, iridoids, amino acid derivatives, alkaloids, polyacetylenes, and quinoids
  • Draws on the unparalleled expertise of an international team of contributors


The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field.

The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products.

Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.


Table of Contents

Overview of the Field of Column High Performance Liquid Chromatography in Phytochemical Analysis and the Structure of the Book, Monika Waksmundzka-Hajnos and Joseph Sherma

Herbal Drugs and the Role of Chromatographic Methods in Their Analysis, Ioanna Chinou

Plant Products in Nutrition and Dietary Supplements: Quality Control, Grażyna Zgórka

HPLC in Chemosystematics, Renate Spitaler and Christian Zidorn

Phytochemistry, Phytopharmacology, and the Biological Role of Plant Metabolites, Krystyna Skalicka-Woźniak, Michał Hanos, and Kazimierz Głowniak

Sample Preparation of Plant Material, Anna Oniszczuk and Anna Hawrył

Stationary Phases and Columns in Analysis of Primary and Secondary Metabolites, Fred Rabel

Separation of Nonionic Analytes: Reversed- and Normal-Phase HPLC, Mirosław Hawrył and Wojciech Markowski

Separation of Ionic Analytes: Reversed-Phase, Ion-Pair, Ion-Exchange, and Ion-Exclusion HPLC, Monika Waksmundzka-Hajnos and Łukasz Cieśla

Gradient Elution and Computer-Assisted Method Development, Mirosław Hawrył

LC-MS as a Method of Identification and Quantification of Plant Metabolites, Gunther Stecher, Robert Mayer, Thomas Ringer, Muhammad A. Hashir, Saowapak Kasemsook, Muhammad N. Qureshi, and Gunther K. Bonn

LC-NMR and Related Techniques for the Rapid Identification of Plant Metabolites, Jean-Luc Wolfender

Photodiode Array (PDA) and Other Detection Methods in HPLC of Plant Metabolites, Wojciech Markowski and Monika Waksmundzka-Hajnos

Quantitative Analysis—Method Validation—Quality Control, Pierre Masson

Confirmation of Chirality of Some Natural Products by the HPLC Method, Beata Polak

Primary Metabolites

HPLC of Carbohydrates, Angelika Koch, Simla Basar, and Rita Richter

HPLC of Plant Lipids, Marek Gołębiowski, Monika Paszkiewicz, Łukasz Haliński, and Piotr Stepnowski

HPLC Analysis of Amino Acids, Peptides, and Proteins, Giuseppe Mennella, Antonietta D'Alessandro, and Gianluca Francese

Secondary Metabolites—Shikimic Acid Derivatives
Application of HPLC in the Analysis of Phenols, Phenolic Acids, and Tannins, Tuulia Hyotylainen and Maarit Kivilompolo

Application of HPLC in Coumarin Analyses, Alev Tosun and Petr Tomek

High Performance Liquid Chromatography of Flavonoids, Monika Waksmundzka-Hajnos, Anna Oniszczuk, Mieczysław Hajnos, and Tomasz Oniszczuk

HPLC of Lignans, Annika I. Smeds

Secondary Metabolites—Isoprenoids
HPLC of Mono- and Sesquiterpenes, Angelika Koch, Simla Basar, and Rita Richter

HPLC Analysis of Diterpenes, Michał Ł. Hajnos

High Performance Liquid Chromatography of Triterpenes (Including Saponins), Wieslaw Oleszek and Anna Stochmal

HPLC of Carotenoids, Łukasz Cieśla

HPLC of Steroids, Laurence Dinan, Juraj Harmatha, and René Lafont

HPLC of Iridoids, Rilka Taskova, Tetsuo Kokubun, and Kalina Alipieva

Secondary Metabolites—Amino Acid Derivatives
HPLC of Indole Alkaloids, Anna Petruczynik

HPLC of Isoquinoline Alkaloids, László Kursinszki, Hajnalka Hank, Ágnes Kéry, and Éva Szőke

HPLC of Tropane Alkaloids, Tomasz Mroczek

HPLC of Alkaloids from the Other Biosynthetic Groups, Jolanta Flieger

Secondary Metabolites—Compounds Derived from Acetogenine (Acetylocoenzyme A)
HPLC Analysis of Polyacetylenes, Lars P. Christensen and Kathrine B. Christensen

HPLC of Quinonoid Phytochemicals, Subhalakshmi Ghosh, Madhushree Das Sarma, and Banasri Hazra


Subscribe to our mailing list

* indicates required


Sold Out