Clinical Applications of Mass Spectrometry Methods and Protocols By Garg, Uttam, Hammett-Stabler, Catherine A. (Eds.)

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Clinical Applications of Mass Spectrometry

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 603

Garg, Uttam, Hammett-Stabler, Catherine A. (Eds.)

2010, XVI, 540p. 264 illus., 132 illus. in color.

 

  • Covers a range of gas chromatography- and liquid chromatography-based mass spectrometry techniques and systems
  • Includes multiple analytical options presented for many analytes
  • Serves as a comprehensive resource of mass spectrometry procedures for the clinical laboratory whether new to MS or experienced
  • Contains well-organized procedures that will greatly facilitate assay implementations
  • Provides a resource for materials and hints for troubleshooting problems
  • Covers a wide range of analytes including hormones, drugs and metabolites

As mass spectrometric methods now offer a level of specificity and sensitivity unrealized by spectrophotometric- and immunoassay-based methods, mass spectrometry has entered the clinical laboratory where it is being used for a wide range of applications. In Clinical Applications of Mass Spectrometry: Methods and Protocols, expert researchers provide detailed step-by-step procedures for the analysis of number of analytes of clinical importance. This versatile and expansive volume covers mass spectrometry methods for analytes including a variety of drugs, hormones, and metabolic compounds spanning the disciplines of toxicology, therapeutic drug monitoring, endocrinology, and pediatric metabolism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the analytes, lists of the necessary materials and reagents, readily reproducible analytical protocols, and detailed notes on troubleshooting and avoiding known pitfalls.

Comprehensive and dependable, Clinical Applications of Mass Spectrometry: Methods and Protocols offers its readers a wide array of valuable methods for experienced mass spectrometric labs that are looking to introduce new analyses as well as for those laboratories currently considering the addition of this resourceful and vital technology.

Content Level » Professional/practitioner

Keywords » Clinical analytes - Drugs - Ethylen - Gas chromatography - Hormones - LC-MS or GC-MS - Liquid chromatography - Mass Spectrometry - Metabolic compounds - indomethacin

 

Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii

1. The Evolution of Mass Spectrometry in the Clinical Laboratory. . . . . . . . . . . . . . . 1
Catherine A. Hammett-Stabler and Uttam Garg

2. Measurement of Plasma/Serum Acylcarnitines Using Tandem
Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Nathalie Lepage and Susan Aucoin

3. Comprehensive Determination of Amino Acids for Diagnosis of Inborn
Errors of Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Dennis J. Dietzen and Annette L. Weindel

4. Identification and Quantitation of Amphetamine, Methamphetamine, MDMA,
Pseudoephedrine, and Ephedrine in Blood, Plasma, and Serum Using Gas
Chromatography-Mass Spectrometry (GC/MS) . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Josh Gunn, Scott Kriger, and Andrea R. Terrell

5. Quantification of Antidepressants Using Gas Chromatography-Mass
Spectrometry. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Ruth E. Winecker

6. Quantitation of Argatroban in Plasma Using Liquid Chromatography
Electrospray Tandem Mass Spectrometry (UPLC-ESI-MS/MS) . . . . . . . . . . . . . . 57
Ross J. Molinaro

7. Quantitation of Amobarbital, Butalbital, Pentobarbital, Phenobarbital, and
Secobarbital in Urine, Serum, and Plasma Using Gas Chromatography-Mass
Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Leonard L. Johnson and Uttam Garg

8. Quantitation of Benzodiazepines in Blood and Urine Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Bruce A. Goldberger, Chris W. Chronister, and Michele L. Merves

9. LC-MS/MS Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum,
Plasma, and Meconium. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Stephanie J. Marin and Gwendolyn A. McMillin

10. Simultaneous Determination and Quantification of 12 Benzodiazepines
in Serum or Whole Blood Using UPLC/MS/MS . . . . . . . . . . . . . . . . . . . . . . . . . 107
Josh Gunn, Scott Kriger, and Andrea R. Terrell

11. Determination of Benzoic Acid in Serum or Plasma by Gas
Chromatography-Mass Spectrometry (GC/MS) . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Jeff M. Knoblauch, David K. Scott, Laurie D. Smith, and Uttam Garg

12. Quantification of Busulfan in Plasma Using Liquid Chromatography
Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS) . . . . . . . . . . . . . . 129
Marion L. Snyder and James C. Ritchie

13. Quantitation of Total 11-Nor-9-Carboxy-Delta 9-Tetrahydrocannabinol
in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS). . . 137
C. Clinton Frazee III, Michael Kiscoan, and Uttam Garg

14. Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and
Cocaethylene in Urine and Blood Using Gas Chromatography-Mass
Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Steven W. Fleming, Amitava Dasgupta, and Uttam Garg

15. Identification and Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene
in Blood, Serum, and Plasma Using Ultra-Performance Liquid Chromatography
Coupled to Tandem Mass Spectrometry (UPLC-MS/MS) . . . . . . . . . . . . . . . . . . 157
Scott Kriger, Josh Gunn, and Andrea R. Terrell

16. Detection and Quantification of Cocaine and Benzoylecgonine in Meconium
Using Solid Phase Extraction and UPLC/MS/MS . . . . . . . . . . . . . . . . . . . . . . . . 165
Josh Gunn, Scott Kriger, and Andrea R. Terrell

17. Diagnosis of Creatine Metabolism Disorders by Determining Creatine
and Guanidinoacetate in Plasma and Urine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Qin Sun and William E. O’Brien

18. Broad Spectrum Drug Screening Using Electron-Ionization Gas
Chromatography-Mass Spectrometry (EI-GCMS) . . . . . . . . . . . . . . . . . . . . . . . . . 187
Judy Stone

19. Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid
Triple Quadrupole Linear Ion Trap Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . 203
Judy Stone

20. High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma
Using LC-MS/MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219
Mark M. Kushnir, Alan L. Rockwood, Bingfang Yue, and A. Wayne Meikle

21. 3-Hydroxy-Fatty Acid Analysis by Gas Chromatography-Mass Spectrometry. . . . . 229
Patricia M. Jones and Michael J. Bennett

22. Quantitation of Fentanyl in Blood and Urine Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 245
Bruce A. Goldberger, Chris W. Chronister, and Michele L. Merves

23. Determination of Total Homocysteine in Plasma Using Liquid
Chromatography Coupled to Tandem Mass Spectrometry (LC/MS/MS) . . . . . . 253
Bruno Casetta

24. Quantitation of Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA)
in Urine Using Gas Chromatography-Mass Spectrometry (GC/MS). . . . . . . . . . . 261
Ryan Allenbrand and Uttam Garg

25. Quantitation of 17-OH-Progesterone (OHPG) for Diagnosis of Congenital
Adrenal Hyperplasia (CAH) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Ravinder J. Singh

26. Determination of Hydroxyurea in Serum or Plasma Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 279
David K. Scott, Kathleen Neville, and Uttam Garg

27. Quantitation of Ibuprofen in Blood Using Gas Chromatography-Mass
Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289
Gerry Huber and Uttam Garg

28. Quantitation of Indomethacin in Serum and Plasma Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 297
Sarah Thomas, Amanda Sutton, and Uttam Garg

29. Quantitation of Iothalamate in Urine and Plasma Using Liquid Chromatography
Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS) . . . . . . . . . . . . . . 307
Ross J. Molinaro and James C. Ritchie

30. Identification and Quantitation of Ketamine in Biological Matrices
Using Gas Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . 317
Timothy P. Rohrig, Megan Gamble, and Kim Cox

31. Measurement of Filter Paper Bloodspot Lead by Inductively Coupled
Plasma-Mass Spectrometry (ICP-MS). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 327
Denise M. Timko and Douglas F. Stickle

32. Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots
Using Tandem Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339
X. Kate Zhang, Carole S. Elbin, Frantisek Turecek, Ronald Scott,
Wei-Lien Chuang, Joan M. Keutzer, and Michael Gelb

33. Gas Chromatography-Mass Spectrometry Method for the
Determination of Methadone and 2-Ethylidene-1,5-Dimethyl-3,
3-Diphenylpyrrolidine (EDDP) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 351
Christine L.H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

34. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)
Method for Monitoring Methotrexate in the Setting of Carboxypeptidase-G2
Therapy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 359
Vikram S. Kumar, Terence Law, and Mark Kellogg

35. Quantitation of Methylmalonic Acid in Serum or Plasma Using Isotope
Dilution-Selected Ion Gas Chromatography-Mass Spectrometry . . . . . . . . . . . . . . 365
Jie Chen and Michael J. Bennett

36. Quantitation of Methylmalonic Acid in Plasma Using Liquid
Chromatography – Tandem Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . 371
Claudine Fasching and Jasbir Singh

37. Monitoring of Mycophenolate Acid in Serum or Plasma Using LC
Tandem Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379
Catherine A. Hammett-Stabler, Diane Ciuffetti Geis, James C. Ritchie,
and Christine Papadea

38. Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids
in Urine, Serum, and Plasma Using LC-MS-MS. . . . . . . . . . . . . . . . . . . . . . . . . . . 389
Bingfang Yue, Mark M. Kushnir, Francis M. Urry,
and Alan L. Rockwood

39. Quantitation of Opioids in Blood and Urine Using Gas Chromatography-Mass
Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399
Bruce A. Goldberger, Chris W. Chronister, and Michele L. Merves

40. Quantitation of Morphine, Codeine, Hydrocodone, Hydromorphone,
Oxycodone, Oxymorphone, and 6-Monoacetylmorphine (6-MAM) in Urine,
Blood, Serum, or Plasma Using Liquid Chromatography with Tandem Mass
Spectrometry Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 411
Tim Dahn, Josh Gunn, Scott Kriger, and Andrea R. Terrell

41. Urine Organic Acid Analysis for Inherited Metabolic Disease Using Gas
Chromatography-Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423
Patricia M. Jones and Michael J. Bennett

42. Identification of Urine Organic Acids for the Detection of Inborn
Errors of Metabolism Using Urease and Gas Chromatography-Mass
Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 433
Stanley F. Lo, Velta Young, and William J. Rhead

43. Quantitation of Orotic Acid in Urine Using Isotope Dilution-Selected
Ion Gas Chromatography-Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . 445
Jie Chen and Michael J. Bennett

44. Quantitation of Oxycodone in Blood and Urine Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 453
Bruce A. Goldberger, Chris W. Chronister, and Michele L. Merves

45. Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 461
Angela M. Ferguson and Uttam Garg

46. Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass
Spectrometry (LC-MS-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 469
Magdalena Korecka and Leslie M. Shaw

47. Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid
Chromatography-Tandem Mass Spectrometry (HPLC-MS-MS) . . . . . . . . . . . . . . 479
Keri J. Donaldson and Leslie M. Shaw

48. Analysis of Testosterone in Serum Using Mass Spectrometry . . . . . . . . . . . . . . . . . 489
Robert L. Fitzgerald, Terrance L. Griffin, and David A. Herold

49. Determination of Tetrahydrozoline in Urine and Blood Using Gas
Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . 501
Judy Peat and Uttam Garg

50. Quantitation of 25-OH-Vitamin D (25OHD) Using Liquid Tandem Mass
Spectrometry (LC-MS-MS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 509
Ravinder J. Singh

51. Identification and Quantitation of Zolpidem in Biological Matrices
Using Gas Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . 519
Timothy P. Rohrig, Lydia A. Harryman, and Melissa C. Norton

52. Identification and Quantitation of Zopiclone in Biological Matrices
Using Gas Chromatography-Mass Spectrometry (GC-MS) . . . . . . . . . . . . . . . . . . 527
Timothy P. Rohrig, Melissa C. Norton, and Lydia A. Harryman

Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 535

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